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目的 :研究脑缺血再灌注后大鼠血脑屏障 (BBB)通透性改变。方法 :采用线栓法大鼠大脑中动脉闭塞的局灶性脑缺血模型 ,缺血 1h后再灌注 ,分别于再灌注后 0h、3h、5h、12h、及 2 4h ,采用免疫组织化学SABC法 ,观察内源性免疫球蛋白G(IgG)在脑组织中的表达。结果 :再灌注 0h、3h时 ,脑组织中未见IgG的表达。再灌注 5h时 ,缺血侧大脑半球纹状体有局灶性的IgG表达。再灌注 12h时 ,缺血侧纹状体及新皮层可见有广泛的IgG的表达。再灌注 2 4h时 ,表达更加明显。结论 :缺血 1h再灌注 3～ 5h时 ,BBB开始受损开放 ,通透性增加。纹状体的BBB较新皮层更易受损。再灌注 12h、2 4h ,外渗的血清蛋白累积增加
Objective: To study the changes of permeability of blood-brain barrier (BBB) in rats after cerebral ischemia-reperfusion. Methods: The focal cerebral ischemia model of middle cerebral artery occlusion (MCAO) was established in rats. The rats were reperfused 1h after ischemia and then were harvested at 0h, 3h, 5h, 12h and 24 h after reperfusion respectively. Immunohistochemical SABC Method to observe the expression of endogenous immunoglobulin G (IgG) in brain tissue. Results: At 0h and 3h after reperfusion, there was no expression of IgG in brain tissue. At 5h after reperfusion, there was focal IgG expression in the ischemic hemisphere striatum. At 12h after reperfusion, a wide range of IgG expression was observed in the ischemic striatum and neocortex. At 24 hours after reperfusion, the expression was more obvious. CONCLUSION: BBB begins to be damaged and open with an increase of permeability at 1h after reperfusion for 3h to 5h. The striatum BBB is more vulnerable than the neocortex. Reperfusion 12h, 24h, extravasation of serum protein accumulation increased