目的 :探讨白介素 5 (IL 5 )及粒细胞 巨噬细胞集落刺激因子 (GM CSF)在哮喘豚鼠支气管肺组织中的表达。方法 :实验分为哮喘组和对照组 ,每组各 15只豚鼠。采用原位杂交方法检测两组豚鼠支气管肺组织的IL 5和GM CSFmRNA表达。结果 :哮喘组支气管肺组织IL 5和GM CSFmRNA表达强度 (细胞数 /HP)分别为 36 0 0± 8 0 0和 32 0 0± 9 0 0 ,对照组分别为 14 0 0± 5 0 0和 18 0 0± 7 0 0 ,哮喘组IL 5和GM CSFmRNA表达强度显著高于对照组 (P <0 0 1)。哮喘组肺组织嗜酸细胞 (EOS)浸润密度 (细胞数 /HP)为 48 0 0± 2 6 0 0 ,对照组为 19 0 0± 6 0 0 ,哮喘组显著高于对照组 (P <0 0 1)。结论 :哮喘豚鼠支气管肺组织IL 5和GM CSFmRNA高表达可能在气道炎症中发挥重要作用。 Objective: To investigate the expression of interleukin-5 (IL-5) and granulocyte-macrophage colony stimulating factor (GM-CSF) in bronchopulmonary tissues of asthmatic guinea pigs. Methods: The experiment was divided into asthma group and control group, with 15 guinea pigs in each group. In situ hybridization was used to detect IL-5 and GM-CSF mRNA expression in bronchial lung tissue of two groups of guinea pigs. Results: The expression of IL-5 and GM-CSF in bronchial lung tissue in asthma group was 36 0 0 ± 0 0 and 32 0 0 ± 9 0 0, respectively, while the control group was 14 0 0 ± 500 and The expression of IL-5 and GM-CSF mRNA in asthma group was significantly higher than that in control group (P <0.01). In the asthma group, the eosinophil (EOS) infiltration density (number of cells / HP) in the lung tissue was 48 0 0 ± 26 0 0 and in the control group was 19 0 0 ± 6 0 0, the asthma group was significantly higher than that of the control group 0 1). Conclusion: The high expression of IL-5 and GM-CSF mRNA in bronchial lung tissue of asthmatic guinea pigs may play an important role in airway inflammation.