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目的 观察不同类型甲状腺癌中p53抑癌基因蛋白的表达。方法 应用LSAB免疫组织化学技术对 113例不同类型甲状腺癌石蜡组织中p53蛋白的表达进行研究。结果 10例低分化甲状腺癌中有 3例 ( 30 % )、9例未分化甲状腺癌中有 6例 ( 67% )呈现p53蛋白染色阳性 ,而乳头状癌 (除 2例伴有低分化区域呈现散在阳性外 )、滤泡型癌中均未见p53蛋白的表达。组织学分析表明 ,肿瘤分化较低的细胞区p53蛋白阳性染色的范围和强度明显高于分化相对高的区域。结论 p53基因突变是甲状腺癌发生、发展中的晚期遗传事件 ,p53蛋白的表达与甲状腺癌的进展、浸润和去分化有关 ,检测该基因蛋白可以作为判断甲状腺癌生物学行为、估价预后的有效参考指标。
Objective To observe the expression of p53 tumor suppressor gene protein in different types of thyroid cancer. Methods LSAB immunohistochemical technique was used to study the expression of p53 protein in paraffin tissues of 113 different types of thyroid cancer. Results 3 out of 10 poorly differentiated thyroid cancers (30%) and 6 (67%) of 9 undifferentiated thyroid cancers stained positive for p53 protein, while papillary carcinomas (except 2 cases with poorly differentiated areas) In sporadic positive cases, no expression of p53 protein was found in follicular carcinoma. Histological analysis showed that the extent and intensity of positive staining of p53 protein in the cell region with lower tumor differentiation was significantly higher than that in the relatively high differentiation region. Conclusion The p53 gene mutation is a late genetic event in the occurrence and development of thyroid carcinoma. The expression of p53 protein is related to the progression, infiltration and dedifferentiation of thyroid carcinoma. The detection of this gene protein can be used as an effective reference for judging the biological behavior and valuing the prognosis of thyroid cancer. index.