论文部分内容阅读
目的探讨慢性应激对大鼠海马长时程增强 (LTP)和氨基酸类神经递质的影响及苯妥英钠对它们的效应。方法将 2 4只SD雄性大鼠随机分为对照组、应激 +生理盐水组和应激 +苯妥英钠组 ,每组 8只。采用离体海马脑片结合电生理的方法观测海马CA1区LTP的变化。以群体峰电位 (PS)的幅值和场兴奋性突触后电位 (fEPSP)的斜率作为观察LTP变化的指标。应用高效液相色谱紫外检测法检测海马氨基酸类神经递质的含量。结果 (1)应激 +生理盐水组PS幅值和fEPSP斜率在高频串刺激后增大的幅度低于对照组和应激 +苯妥英钠组 (P <0 .0 5 )。 (2 )应激 +生理盐水组和应激+苯妥英钠组的天冬氨酸含量 [分别为 (4 .746± 0 .60 9) μmol/g和 (4 .948± 0 .75 1) μmol/ g]高于对照组 [(2 .42 5± 0 .2 11)μmol/ g ,P <0 .0 1] ;应激 +生理盐水组的谷氨酸含量 [(8.0 94± 1.0 3 5 ) μmol/ g]高于对照组 [(6.0 16± 0 .677) μmol/g]和应激 +苯妥英钠组 [(6.970± 0 .64 7) μmol/g] ;P <0 .0 5 ;应激 +苯妥英钠组的GABA含量 [(5 .14 2± 0 .662 ) μmol/g]高于对照组 [(4 .2 2 9± 0 .44 9) μmol/ g]和应激 +生理盐水组 [(4 .2 49± 0 .463 ) μmol/g] ,P <0 .0 1。结论慢性应激使大鼠海马CA1区LTP的形成受抑制 ,天冬氨酸和谷氨酸?
Objective To investigate the effect of chronic stress on long-term potentiation (LTP) and amino acid neurotransmitter in hippocampus of rats and the effect of phenytoin on them. Methods Twenty-four SD male rats were randomly divided into control group, stress + saline group and stress + phenytoin sodium group, with 8 rats in each group. The changes of LTP in the hippocampal CA1 region were observed by using the hippocampal slices combined with electrophysiological methods. The amplitude of population peak potential (PS) and the slope of the field excitatory postsynaptic potential (fEPSP) were used as indices for the observation of changes in LTP. The contents of amino acid neurotransmitters in the hippocampus were detected by high performance liquid chromatography with ultraviolet detection. Results (1) PS amplitude and fEPSP slope of stress + saline group increased less than that of control group and stress + phenytoin sodium group (P <0.05) after high frequency stimulation. (2) Aspartate content in stress + saline group and stress + phenytoin group [(4.746 ± 0.609) μmol / g and (4.948 ± 0.751) μmol / g] was higher than that in the control group [(2.42 5 ± 0.21 1 μmol / g, P <0.01]; the glutamate content in stress + saline group [(8.094 ± 1.03 5 ) (μmol / g) were higher than those in the control group [(6.0 16 ± 0.677 μmol / g)] and phenytoin sodium group [(6.970 ± 0.647) μmol / g] The levels of GABA in the stress + phenytoin group were significantly higher than those in the control group [(4.22 ± 0.449) μmol / g] [(5.214 ± 0.662) μmol / g] Saline group [(4 .249 ± 0 .463) μmol / g], P <0.01. Conclusion Chronic stress inhibits the formation of LTP in hippocampal CA1 region, aspartate and glutamate?