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目的研究分别含营养肉汤和生理盐水的中和剂对使用中消毒剂染菌量检测结果的影响,以指导消毒剂监测过程中正确选择采样液、保存条件及送检时间。方法配制以营养肉汤和生理盐水为底物、有效成分为硫代硫酸钠的2种中和剂(底物为营养肉汤的为中和剂1,底物为生理盐水的为中和剂2),对不同时间及温度下消毒后的大肠埃希菌变化情况进行分析。结果在未经消毒的情况下,中和剂1和中和剂2中的大肠埃希菌在6 h内均发生明显的增菌变化,不同时间点的菌落数差异均有统计学意义(均P<0.01)。在36℃温度条件下,2种中和剂经消毒处理后不同时间的大肠埃希菌菌落数差异均有统计学意义(均P<0.01),中和剂1在5 h开始菌落数与0 h的差异具有统计学意义(均P<0.01)。其他2种温度条件下不同时间点的大肠埃希菌菌落数差异无统计学意义(均P>0.05)。结论在进行使用中消毒剂的采样中,生理盐水较营养肉汤更加适合作为采样液复方中和剂底物,而且采样后应在4 h内送检,否则应冷藏保存。
Objective To study the effect of neutralizing agent containing nutrient broth and saline on the results of the inoculation of disinfectant in order to guide the correct choice of sample solution, storage conditions and inspection time during disinfectant monitoring. Methods Nutritional broth and saline as substrate, the active ingredient is sodium thiosulfate two kinds of neutralizer (nutrient broth substrate for the neutralizer 1, the substrate for the physiological saline as a neutralizer 2) to analyze the changes of Escherichia coli after disinfection under different time and temperature. Results In the case of non-sterilized, Escherichia coli in Neutralizer 1 and Neutralizer 2 showed significant increase in bacteria within 6 h, and there were significant differences in the number of colonies at different time points P <0.01). At 36 ℃, there were significant differences in the number of Escherichia coli colonies between the two neutralizers after disinfection (P <0.01) h difference was statistically significant (all P <0.01). There were no significant differences in the number of Escherichia coli colonies at the other two temperature conditions (all P> 0.05). Conclusion In the use of disinfectant sampling, physiological broth than nutrient broth is more suitable as a sample solution compound neutralizer substrate, and sampling should be submitted within 4 h, otherwise it should be refrigerated.