目的:在四川5.12抗震救灾时期,开展了灾区生活饮用水的卫生监测工作,由于时间和人力资源的限制,对微生物快速检测提出了要求。方法:运用酶底物法快速检测生活饮用水中三项微生物指标:大肠菌群、耐热大肠菌群、大肠埃希菌。结果:共检测灾区生活饮用水2000余件,其中市政供水350余件,合格率约94%;乡镇供水1650余件,合格率约90%。酶底物法24 h可报告结果,并能同时检测2个指标,实现了快速检测的目的。结论:酶底物法作为快速检测技术,在此次生活饮用水监测工作中发挥了突出的作用,极大地节约了时间和人力,有力地支持了抗震救灾工作的顺利实施,保障了广大灾区人民群众生活饮用水的供水安全。 OBJECTIVE: To carry out sanitary monitoring of drinking water in quake-hit areas during the period of 5.12 earthquake relief in Sichuan Province. Due to the limitation of time and human resources, the requirements for rapid detection of microorganisms are put forward. Methods: Enzyme substrate method was used to rapidly detect three microbial indicators in drinking water: coliform bacteria, heat-resistant coliform bacteria and Escherichia coli. Results: A total of more than 2000 pieces of drinking water were tested in disaster-hit areas. Among them, more than 350 pieces of municipal water were supplied, with a pass rate of 94% and a supply of 1650 pieces of water in villages and towns with a passing rate of 90%. Enzyme substrate method 24 h reportable results, and can simultaneously detect two indicators, to achieve the purpose of rapid detection. Conclusion: The enzyme substrate method, as a rapid detection technique, has played an outstanding role in this drinking water monitoring and greatly saves time and manpower. It has effectively supported the smooth implementation of earthquake relief work and ensured the protection of people in disaster areas Water supply for people’s drinking water is safe.