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目的研究异种抗原免疫及瘤内注射激活的脾淋巴细胞对小鼠肉瘤S180细胞的体外杀伤活性。方法分别用两种异种抗原(灭活链球菌和A型血型抗原)进行试验。将异种抗原腹腔注射全身免疫小鼠,接种S180细胞于小鼠背部,成瘤后,于瘤内注射异种抗原,并设异种抗原仅全身免疫组、异种抗原仅瘤内注射组和空白对照组。用MTT比色法测定小鼠脾淋巴细胞对S180细胞的杀伤率。结果在效靶比20:1时,灭活链球菌全身免疫及瘤内注射组脾淋巴细胞对S180细胞的杀伤率为63.17%±3.94%,显著高于灭活链球菌仅全身免疫组、灭活链球菌仅瘤内注射组和空白对照组(杀伤率分别为10.26%±1.95%、33.30%±3.82%和9.85%±2.76%),P值均<0.05。同时A型血型抗原全身免疫及瘤内注射组脾淋巴细胞对S180细胞的杀伤率为76.27%±4.07%,显著高于A型血型抗原仅全身免疫组、A型血型抗原仅瘤内注射组和空白对照组(杀伤率分别为10.55%±2.29%、40.92%±4.98%和9.85%±2.76%),P值均<0.05。另外A型血型抗原全身免疫及瘤内注射组的杀伤率高于灭活链球菌全身免疫及瘤内注射组,A型血型抗原仅瘤内注射组的杀伤率也高于灭活链球菌仅瘤内注射组,P值均<0.05。结论异种抗原免疫及瘤内注射后小鼠的免疫功能有所提高,激活的脾淋巴细胞对S180细胞有很强的杀伤活性。
Objective To study the in vitro killing activity of murine sarcoma S180 cells by xenoantigen-immunized and splenic lymphocytes activated by intratumoral injection. Methods Two different antigens (inactivated streptococcus and type A blood group antigen) were tested separately. Allogeneic antigens were intraperitoneally injected into mice immunized with S180 cells in the back of the mice. After tumorigenesis, allogeneic antigens were injected intraperitoneally into the tumor and allogeneic antigens were given only to the whole body and allogeneic antigens were injected into the tumor and blank control groups. The killing rate of mouse splenic lymphocytes on S180 cells was determined by MTT assay. Results When the ratio of target to target was 20: 1, the killing rate of splenic lymphocytes to S180 cells was 63.17% ± 3.94%, which was significantly higher than that of systemic immunization with streptococcus inactivated The live streptococcal group and the blank control group (10.26% ± 1.95%, 33.30% ± 3.82% and 9.85% ± 2.76%, respectively) had P <0.05. At the same time, the killing rate of splenic lymphocytes on S180 cells was 76.27% ± 4.07%, which was significantly higher than that of systemic immunization of type A blood group antigens and type A blood group antigens alone The blank control group (killing rates were 10.55% ± 2.29%, 40.92% ± 4.98% and 9.85% ± 2.76%, P <0.05 respectively). In addition, systemic immunization of type A blood group antigen and intratumoral injection group were higher than that of systemic immunization and intratumoral injection group. The killing rate of type A blood group antigen in intratumoral injection group was also higher than that of inactivated tumor group Intra-injection group, P <0.05. Conclusion The immune function of mice after immunization and intratumoral injection is improved. The activated lymphocytes have strong killing activity on S180 cells.