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将甲型肝炎病毒(HAV)核壳蛋白VP1和VP3的6个重叠的亚基因互补(cDNA)片段插入表达载体pBD或pUR中,转入大肠杆菌,在含有异丙基硫代半乳糖苷(IPTG)诱导剂的培养基中培养,表达出6个β-半乳糖苷酶-HAV融合蛋白。这些融合蛋白水溶性差,不能用放射免
Six overlapping subgenomic complementary (cDNA) fragments of the hepatitis A virus (HAV) nucleocapsid proteins VP1 and VP3 were inserted into the expression vector pBD or pUR, transformed into E. coli and transformed in E. coli containing isopropylthiogalactoside IPTG) inducer, 6 β-galactosidase-HAV fusion proteins were expressed. These fusion proteins are poorly water-soluble and can not be radioed-free