从土壤肥力与微生物因子探索连茬障碍机理,以期为其提供科学依据。研究草莓番茄轮作(RST)、番茄连作4年(CT4)和番茄连作10年(CT10)3种蔬菜种植模式根际与非根际土壤微生物区系及生理菌群,并对土壤肥力与微生物生物因子进行主成分分析。结果表明根际土壤微生物三大类群和生理菌群数量均高于非根际,根际效应显著。番茄连作根际与非根际土壤细菌和放线菌数量呈现先增加后减少趋势;真菌数量呈线型增加趋势,CT4和CT10在根际与非根际较RST分别增加9.09%和2.11%、75.48%和57.72%。番茄连作根际土壤硝化细菌和好气性自生固氮菌数量的减少,氨化细菌与好气性纤维素分解菌在短期连作表现为增加长期减少的变化趋势;解钾菌、无机磷和有机磷细菌数量在根际与非根际土壤均减少。在6种研究的种植模式中,RST根际土壤状况最好,其次为CT4的根际与RST非根际土壤,CT10的根际土壤、CT4与CT10非根际土壤状况最差。结论是蔬菜连作造成土壤质量下降,连作年限越长下降越显著。 Exploring the obstacle mechanism of continuous cropping from soil fertility and microbial factors in order to provide a scientific basis for it. The rhizosphere and non-rhizosphere soil microbial flora and physiological microflora of three vegetable planting models including strawberry rotation tomato (RST), tomato continuous crop four years (CT4) and tomato continuous crop ten years (CT10) were studied. The effects of soil fertility and microbial biomass Factor for principal component analysis. The results showed that rhizosphere soil microbial populations were higher than those in the non-rhizosphere soil and rhizosphere. The number of bacteria and actinomycetes in the rhizosphere soil and non-rhizosphere soil of tomato were increased at first and then decreased. The number of fungi increased linearly. The CT4 and CT10 increased by 9.09% and 2.11% 75.48% and 57.72% respectively. The number of nitrifying bacteria and aerobic autotrophic azotobacteria in tomato continuous cropping rhizosphere soil decreased, and the number of ammonifying bacteria and aerobic cellulolytic bacteria showed a long-term decreasing trend in short-term continuous cropping. The quantity of potassium-forcing bacteria, inorganic phosphorus and organic phosphorus bacteria In the rhizosphere and non-rhizosphere soil were reduced. In the six planting models, the rhizosphere soil was the best, followed by CT4 rhizosphere and RST non-rhizosphere soil. CT10 rhizosphere soil and CT4 and CT10 non-rhizosphere soil were the worst. The conclusion is that the quality of soil caused by vegetable continuous cropping decreased, and the longer the continuous cropping age, the more significant decline.