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目的探讨压力蒸汽灭菌的监测方法和影响检测效果的因素。方法采用嗜热脂肪杆菌芽孢菌片试培养,将菌片试管放于标准生物监测包中心部位,监测包置于灭菌器底层排气口上方和进气口2个监测点,经一个灭菌周期、取出监测包中的菌片试管送微生物室培养。结果 1号灭菌器排气口监测点48次监测,1次不合格,合格率97%。进气口监测点合格率100%。2号灭菌器排气口、进气口监测点合格率均为100%。结论医院供应室应高度重视,完善监测措施,规范生物监测,确保所供应的无菌物品绝对安全合格,确保消毒灭菌质量。
Objective To investigate the methods of pressure steam sterilization monitoring and the factors influencing the test results. Methods Bacillus stearothermophilus test culture, the bacterial test tube placed in the center of the standard biological monitoring package, the monitoring package placed on the bottom of the sterilizer and the air inlet above the mouth of the two monitoring points, a sterilization Cycle, remove the monitoring tube in the test tube microbubbles sent to the culture room. Result No. 1 sterilizer vent monitoring point monitoring 48 times, 1 failed, the passing rate of 97%. Air inlet monitoring point pass rate of 100%. 2 sterilizer exhaust port, air intake monitoring points pass rate of 100%. Conclusion The hospital supply room should attach great importance to improve the monitoring measures to standardize biological monitoring to ensure that the supply of sterilized items absolutely safe and qualified to ensure the quality of disinfection and sterilization.