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目的探讨纤维支气管镜(纤支镜)活检组织标本中干细胞标志物Musashi2 mRNA的检测在肺癌中的诊断价值。方法采用RT-PCR技术检测112例肺癌患者和18例良性肺疾病患者纤支镜活检标本中Musashi2 mRNA的表达水平,并分析其在肺癌诊断中的潜在价值。结果 Musashi2 mRNA在肺癌组织中的表达量显著高于非癌肺组织(P<0.05)。ROC曲线下面积为0.759,临界值取0.596时,其敏感度和特异度分别为73.2%和77.8%。即使纤支镜活检组织病理检查未找到癌细胞,Musashi2 mRNA的诊断敏感度仍有69.2%。结论纤支镜活检组织标本Musashi2 mRNA可能是肺癌诊断的有效指标。
Objective To investigate the diagnostic value of Musashi2 mRNA in lung cancer by fiberoptic bronchoscopy (biopsy) biopsy specimens. Methods Musashi2 mRNA expression was detected by RT-PCR in 112 lung cancer patients and 18 patients with benign lung disease. The potential value of Musashi2 mRNA in lung cancer was analyzed. Results The expression of Musashi2 mRNA in lung cancer tissues was significantly higher than that in non-cancerous lung tissues (P <0.05). The area under the ROC curve was 0.759, and when the cutoff value was 0.596, the sensitivity and specificity were 73.2% and 77.8% respectively. The diagnostic sensitivity of Musashi2 mRNA was 69.2% even though no cancerous cells were found by biopsy. Conclusion Musashi2 mRNA in biopsy specimens may be an effective diagnostic indicator for lung cancer.