论文部分内容阅读
目的：比较同种异体阔筋膜在不同保存方法下生物学性能的改变，为临床应用提供依据。方法取新鲜同种异体阔筋膜，随机分成三组保存：Ⅰ组梯度乙醇固定保存，Ⅱ组深低温甘油保存，Ⅲ组复合抗生素溶液浅低温保存。于保存后不同时段检测理化特性和生物力学性能，观察其变化，以新鲜阔筋膜作为对照。结果Ⅰ组：保存1个月、3个月时阔筋膜理化特性正常，保存6个月时阔筋膜表面色泽及柔韧性能发生改变，HE 染色结果显示，随着保存时间的延长，纤维排列密度逐渐降低。Ⅱ组和Ⅲ组：保存1个月、3个月和6个月时，阔筋膜理化特性均正常。三种方法保存6个月时生物力学性能与新鲜阔筋膜比较无统计学差异。结论三种保存方法均可用于同种异体阔筋膜的保存，在观察期内生物力学性能未见显著性变化。梯度乙醇固定保存在6个月后有形态学变化，深低温甘油保存和复合抗生素溶液浅低温保存则未观察到变化。长时间、大量保存同种异体阔筋膜时不建议使用梯度乙醇固定保存法。“,”Objective To compare the biological property changes of allogeneic broad fascia preserved with different methods and provide guidance for clinical application.Methods Fresh allogeneic broad fascia was randomly divided into three groups according to storage methods:groupⅠwas preserved in gradient alcohol,group Ⅱ in glycerin under deep low temperature and group Ⅲ in composite antimicrobial solution under mildly low temperature.Changes of the physical,chemical and biomechanical performance were observed in three groups.The fresh broad fascia was used as control.Results GroupⅠhad the same physical and chemical properties as fresh broad fascia after 3 months preservation, while the color and flexibility changed after being stored for 6 months.HE staining showed that the fiber density decreased gradually with the extension of storage time.Group Ⅱ and group Ⅲ had the similar physical and chemical properties as fresh broad fascia after being preserved for 6 months.There was no statistical difference of biomechanics in 3 groups after 6 months observation compared with fresh broad fascia.Conclusion All of the three methods can be used to preserve al-logeneic broad fascia.However,the method of gradient alcohol is not recommended to use in the long-term or large-quantity storage of allogeneic broad fascia.