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对采自江苏省姜堰、靖江和常州3个地区的水稻恶苗病样品进行了病原菌的分离和鉴定,利用菌丝生长速率法测定了病原菌对咪鲜胺和氰烯菌酯的敏感性。结果表明:分离得到77株水稻恶苗病菌Fusarium fujikuroi;咪鲜胺对水稻恶苗病菌的EC50值在0.020~1.333μg/m L之间,分离到的菌株对咪鲜胺均表现为中抗和高抗,其中中抗菌株18株,占23.38%,高抗菌株59株,占76.62%,说明咪鲜胺高抗菌株已经成为江苏省的恶苗病菌优势群体。氰烯菌酯(JS399-19)对水稻恶苗病菌的EC50值在0.012~2.040μg/m L之间,菌株的敏感性频率近似正态分布,EC50均值为(0.684±0.265)μg/m L,建议将此值作为江苏省水稻恶苗病菌对氰烯菌酯的敏感性基线。
The pathogenic bacteria were isolated and identified from the samples of rice yellows from Jiangyan, Jingjiang and Changzhou in Jiangsu Province. The mycelial growth rate was used to determine the sensitivity of the pathogen to prochloraz and cyanogenic insecticides. The results showed that 77 strains of Fusarium fujikuroi were isolated and the EC50 value of prochloraz against Fusarium oxysporum was between 0.020 and 1.333 μg / mL. The isolates exhibited moderate resistance to prochloraz and Among them, 18 were medium-resistant strains, accounting for 23.38%, and 59 were high-resistant strains, accounting for 76.62% of the total, indicating that prochloraz antibacterial strains have become the dominant group of Bacillus thuringiensis in Jiangsu Province. The EC50 values of JS399-19 against Fusarium oxysporum in rice ranged from 0.012 to 2.040 μg / mL. The frequency of susceptibility of strain JS399-19 was approximately normal distribution with EC50 of (0.684 ± 0.265) μg / m L , It is recommended that this value be used as the baseline for the sensitivity of rice Foki to tanshinone in Jiangsu Province.