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目的:建立大鼠早孕期戊酸雌二醇用药模型,从肛门生殖器距离(AGD)、附睾、睾丸组织发育等方面评估戊酸雌二醇对雄性子代生殖系统发育的影响。方法:妊娠SD大鼠随机分对照组及低、中、高剂量组,孕6～15d每天分别给予戊酸雌二醇0、0.2、0.5、0.8mg/kg灌胃,分娩后正常喂养其雄性子代,出生3d、出生21d分别测量雄性子代AGD,出生60d测量附睾、睾丸的脏器系数(附睾、睾丸重量g/大鼠体重100g),进行睾丸组织学切片观察生精小管的形态学变化,测量生精小管直径和上皮高度。结果:对照组及各给药组雄性子代出生3d、出生21d的AGD比较,无明显差异(P>0.05);对照组及各给药组子代出生60d的附睾、睾丸脏器系数比较、睾丸生精小管直径及上皮高度比较,均无明显差异(P>0.05)。结论:早孕期大鼠补充一定剂量(0.2～0.8mg/kg)戊酸雌二醇,出生的雄性子代在生殖系统发育上未受明显影响,性成熟期的睾丸组织学未见明显改变。
OBJECTIVE: To establish a rat model of estradiol valerate during early pregnancy and to evaluate the effects of estradiol valerate on the reproductive development of male offspring in terms of anal genital distance (AGD), epididymis, testicular tissue development and so on. Methods: Pregnant SD rats were randomly divided into control group and low, medium and high dose groups. The rats were orally administered 0,0.2,0.5,0.8mg / kg estradiol valerate daily from 6th to 15th day of gestation. The males The male offspring AGD were measured on the 3rd day of birth and on the 21st day of birth, and the organ coefficient of the epididymis and testis (epididymis, testis weight g / rat body weight 100g) was measured 60 days after birth to observe the morphology of seminiferous tubules Variations were measured for seminiferous tubule diameter and epithelial height. Results: There was no significant difference in the AGD of the male offspring between the control group and each administration group on the 3rd day of birth and on the 21st day of birth (P> 0.05) Testicular seminiferous tubule diameter and epithelial height, there was no significant difference (P> 0.05). CONCLUSION: In the first trimester of pregnancy, a dose of 0.2 ~ 0.8 mg / kg of estradiol valerate was added to the male offspring. The male offspring did not significantly affect the reproductive development of the reproductive system. No significant changes were found in the testis histology of the sexual maturity.