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天然磷脂是一类含磷酸根的类脂化合物的总称,在医药学、营养学、化妆品和生命科学领域等有重要的应用价值。本文综述了不同原料样品中磷脂种类和其各分子的色谱法分离、纯化。着重讨论高效液相色谱法分离技术及其影响因素。最后概述了有助于提高分离选择性、检测灵敏度的化学衍生化方法。
Natural phospholipids are a group of phosphate-containing lipid compounds in general, in the fields of medicine, nutrition, cosmetics and life sciences have important application value. This review summarizes the separation and purification of phospholipid species and their molecules by different raw materials. Focus on high performance liquid chromatography separation technology and its influencing factors. Finally, the chemical derivatization methods that help to improve separation selectivity and detection sensitivity are summarized.