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目的：观察内皮素（ＥＴ）在急性缺血性肾损伤后的变化及异搏定对肾的保护作用和环孢素Ａ（ＣｓＡ）肾毒性与ＥＴ之间的关系。方法：通过阻断大鼠肾动脉６０分钟造成急性缺血性肾功能衰竭（ＩＡＲＦ）模型，用放射免疫方法测定肾脏缺血再灌注１小时、６小时和２４小时血浆及肾组织中ＥＴ的变化；同时观察异搏定和ＣｓＡ对ＥＴ肾功能的影响。结果：ＩＡＲＦ时，血浆及肾组织中ＥＴ在３个时间点均有不同程度的升高；异搏定可降低ＩＡＲＦ大鼠血浆和肾组织中的ＥＴ含量；ＣｓＡ引起ＥＴ活性进一步升高，并加重肾损伤。结论：ＥＴ可能是导致ＩＡＲＦ的一个重要因子；异搏定能抑制ＩＡＲＦ时ＥＴ的分泌和释放而保护肾脏；ＣｓＡ的肾毒性可能与其诱导ＥＴ的分泌和释放有关。
Objective: To observe the changes of endothelin (ET) after acute ischemic renal injury and the protective effect of verapamil on the kidney and the relationship between cyclosporine A (CsA) nephrotoxicity and ET. Methods: Acute Ischemic Renal Failure (IARF) model was established by blocking the renal arteries of rats for 60 minutes. The changes of ET in plasma and renal tissues at 1 hour, 6 hours and 24 hours after renal ischemia-reperfusion were determined by radioimmunoassay At the same time, we observed the effects of verapamil and CsA on the renal function of ET. Results: In IARF, plasma ET and ET were increased in varying degrees at three time points. Verapamil decreased ET content in plasma and kidney of IARF rats. CsA induced a further increase in ET activity and Increased kidney damage. Conclusion: ET may be an important factor leading to IARF. Verapamil may inhibit the secretion and release of ET in IARF to protect the kidney. The nephrotoxicity of CsA may be related to the secretion and release of ET.