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目的 探讨持续快速心房起搏对犬肺静脉和心房组织连接蛋白 43(Cx43)和Ⅲ型胶原的影响。方法 16只杂种犬,随机分为持续快速心房起搏组(8只)和正常对照组 (8只 ),前者以 400次 /min的频率持续起搏 10周,建立心房颤动(房颤)动物模型。分别取两组犬的左上肺静脉、左房游离壁和右心耳等部位的心肌组织进行Cx43的免疫荧光半定量分析和Ⅲ型胶原纤维定量分析。结果10周后快速心房起搏组所有犬均可诱发出持续性房颤。快速心房起搏组犬肺静脉、左房游离壁和右心耳部位的Cx43水平显著高于正常对照组犬各相应的部位 (肺静脉: 3370 .91±275. 11与1405 .82±90. 38, P<0. 05;左房游离壁: 2448. 68±272 .10与 1467. 12±147 .93,P<0. 05;右心耳: 2331 .96±199 .61与 1288. 27±216 .22, P<0 .05)。快速心房起搏组犬肺静脉Cx43的水平显著高于左心房游离壁和右心耳(P<0. 05),而左心房和右心耳部位的Cx43水平差异无统计学意义 (P>0. 05)。持续快速心房起搏组犬肺静脉、左房游离壁和右心耳等部位的Ⅲ型胶原含量显著高于正常对照组犬各相应部位(肺静脉: 3301 97±309 70与 1404 56±178 02, P<0 05;左房游离壁: 2477 86±190. 43与1479. 20±187 .17, P<0 .05;右心耳: 2045 .92±139 .43与 1417. 07±139. 43,P<0 .05 )
Objective To investigate the effect of continuous rapid atrial pacing on pulmonary vein and atrial tissue connexin 43 (Cx43) and type Ⅲ collagen in dogs. Methods Sixteen dogs were randomly divided into continuous atrial fibrillation (AF) group (n = 8) and normal control group (n = 8). The former was given pacing for 400 times / min for 10 weeks. model. Two groups of canine left ventricular pulmonary veins, left atrial free wall and right atrial appendage and other parts of the myocardial tissue Cx43 semi-quantitative immunofluorescence and type Ⅲ collagen fibers quantitative analysis. Results After 10 weeks of rapid atrial pacing group, all dogs were able to induce persistent atrial fibrillation. Fasting atrial pacing group canine pulmonary vein, left atrial free wall and right atrial appendage Cx43 levels were significantly higher than the normal control group, the corresponding parts of the dog (pulmonary vein: 3370.91 ± 275.11 and 1405.82 ± 90.38, P <0 05; left atrium free wall: 2448. 68 ± 272 .10 and 1467. 12 ± 147 .93, P <0 05; right atrial appendage: 2331 .96 ± 199 .61 and 1288. 27 ± 216 .22 , P <0 .05). The level of Cx43 in the pulmonary vein of patients with rapid atrial pacing was significantly higher than that of the left atrial free wall and the right atrial appendage (P <0.05), while there was no significant difference in the Cx43 level between the left atrium and right atrial appendage (P> 0.05) . Sustained rapid atrial pacing group canine pulmonary vein, left atrial free wall and right atrial appendage and other parts of the type Ⅲ collagen content was significantly higher than the normal control group of dogs corresponding parts (pulmonary vein: 3301 97 ± 309 70 and 1404 56 ± 178 02, P < 0 05; left atrium free wall: 2477 86 ± 190.43 and 1479.20 ± 187.17, P <0.05; right atrial appendage: 2045.92 ± 139.43 and 1417.07 ± 139.43, P < 0 .05)