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目的：观察丙酮氰醇对离体红细胞膜的急性损害作用。方法：将完整的红细胞和分离的红细胞膜分别与不同浓度丙酮氰醇，温育不同时间。结果：发现两者膜的脂质过氧化（ＴＢＡＲＳ）水平增高，钠钾ＡＴＰ酶的活性降低，荧光偏振度升高（即膜流动性降低），和对照组比较都具有显著性差别，并存在剂量反应关系和时间反应关系。胆固醇（Ｃ）／磷脂（ＰＬ）的比值和对照组比较有显著性差别。相关分析表明：膜的脂质过氧化水平（ＴＢＡＲＳ）和钠钾ＡＴＰ酶的活性有明显的负相关，膜的脂质过氧化水平与荧光偏振度有明显的相关，荧光偏振度和钠钾ＡＴＰ酶有明显的相关。荧光偏振度（Ｐ）与胆固醇／磷酯的比值没有相关。结论：红细胞膜可能是丙酮氰醇作用的一个重要的靶器官，膜的脂质过氧化可能是造成细胞膜结构和功能改变的一个重要原因。
Objective: To observe the acute damage of erythrocyte membrane by acetone cyanogen alcohol. Methods: The intact erythrocytes and isolated erythrocyte membranes were incubated with different concentrations of acetone cyanogen alcohol for different time. Results: The levels of lipid peroxidation (TBARS) and Na + -K + -ATPase activity of the two membranes were increased, and the degree of fluorescence polarization increased (ie, the membrane fluidity decreased) compared with the control group, and there was a significant difference Dose-response relationship and time-response relationship. Cholesterol (C) / phospholipid (PL) ratio and the control group there is a significant difference. Correlation analysis showed that the membrane lipid peroxidation level (TBARS) and sodium potassium ATPase activity has a significant negative correlation, membrane lipid peroxidation level and fluorescence polarization degree has a significant correlation, fluorescence polarization and sodium potassium ATP Enzymes have obvious relevance. There was no correlation between fluorescence polarization (P) and cholesterol / phospholipid ratio. CONCLUSION: Erythrocyte membrane may be an important target organ for the action of acetone cyanohydrin. Lipid peroxidation of membrane may be an important reason for the change of cell membrane structure and function.