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目的 揭示胃癌与端粒酶活性及DNA倍体的关系。方法 检测 30例胃癌标本 ,同时取无瘤残端作为对照。端粒酶检测采用端粒重复扩增 酶联免疫吸附法 (TRAP ELISA法 )。DNA倍体的测定采用流式细胞术 ,一步法检测DNA含量。结果 肿瘤瘤体端粒酶阳性率 83 3% (2 5 / 30 ) ,无瘤残端端粒酶阳性率 3 3%(1/ 30 ) (P <0 .0 5 ) ;端粒酶阳性瘤体平均直径 6 5cm ,阴性瘤体平均直径 3 6cm(P <0 .0 5 ) ;端粒酶阳性肿瘤淋巴转移率 5 3 3% (16 / 30 ) ,阴性者无淋巴转移 (0 / 5 ) (P <0 .0 5 ) ;端粒酶阳性肿瘤中异倍体肿瘤占 5 6 0 % (14/2 5 ) ,而端粒酶阴性者无异倍体出现 (P <0 .0 5 )。结论 胃癌端粒酶活性升高 ,端粒酶阳性肿瘤瘤体大 ,淋巴转移率高 ,且异倍体发生率高 ,预后差。提示端粒酶的激活与胃癌的发生发展有密切关系。
Objective To reveal the relationship between gastric cancer and telomerase activity and DNA ploidy. Methods 30 cases of gastric cancer specimens were detected, at the same time take the tumor-free stump as a control. Telomerase detection using telomeric repeat amplification enzyme-linked immunosorbent assay (TRAP ELISA). DNA ploidy determination by flow cytometry, one-step method to detect DNA content. Results The positive rate of telomerase in tumors was 83 3% (25/30), and the positive rate of telomerase in tumor-free stumps was 33% (1/30) (P <0.05). The positive rate of telomerase positive tumors The average diameter of the tumor was 65 cm, the average diameter of the negative tumor was 36 cm (P <0.05), the rate of lymphatic metastasis of the telomerase positive tumor was 53.3% (16/30) (P <0.05). In the telomerase positive tumors, aneuploid tumors accounted for 56.0% (14/2 5), while those with telomerase negative had no aneuploidy (P <0.05) . Conclusion The telomerase activity of gastric cancer is high, telomerase positive tumor is large, lymphatic metastasis rate is high, aneuploidy is high, and the prognosis is poor. Tip telomerase activation and the occurrence and development of gastric cancer are closely related.