从卵清蛋白免疫的SD大鼠胸腹腔中采集的肥大细胞,当以相同的变应原触发时引起肥大细胞释放组胺。用改良的荧光法测定组胺量,计算肥大细胞组胺释放率,实验结果表明,卵清蛋白免疫大鼠第3周肥大细胞组胺释放率达到高峰,在一定范围内,组胺释放率随变应原浓度增加而升高。绵羊抗大鼠IgE抗血清对正常大鼠肥大细胞亦有触发作用。这一试验可用于速发型过敏反应和抗过敏药物的研究。 Mast cells collected from the peritoneal cavity of ovalbumin-immunized SD rats cause mast cells to release histamine when triggered with the same allergen. The amount of histamine was measured by modified fluorescence method, and the histamine release rate was calculated. The results showed that the histamine release rate of mast cells reached the peak in the 3rd week after ovalbumin immunization, and the release rate of histamine Allergen concentration increased and increased. Sheep anti-rat IgE antiserum also triggers normal mast cells. This test can be used for rapid-onset allergic reactions and allergy drug research.