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在室内用甲氧虫酰肼对甜菜夜蛾Spodoptera exigua(Hübner)幼虫进行抗药性选育,经过13代汰选,甜菜夜蛾抗性种群(Met-R)对甲氧虫酰肼的抗性倍数为4.19倍;交互抗性测定发现,Met-R甜菜夜蛾对毒死蜱、高效氯氰菊酯、虫螨腈、虫酰肼、甲胺基阿维菌素苯甲酸盐等药剂的交互抗性比值在1.05～2.07之间,不存在交互抗性。离体酶活性测定结果表明,Met-R甜菜夜蛾5龄幼虫中肠谷胱甘肽S-转移酶比活力是相对敏感种群(SS)甜菜夜蛾的2.44倍,存在显著性差异,说明谷胱甘肽S-转移酶活性增强可能与甜菜夜蛾对甲氧虫酰肼产生抗药性有关;但Met-R甜菜夜蛾全酯酶催化活性与SS的无显著性差异,说明其抗药性可能与酯酶无关。
Insect-resistant breeding of Spodoptera exigua (Hübner) larvae was conducted with methoxyfenozide in the laboratory. After 13 generations of selection, the resistant population of Metarhizobium moth (Met-R) to methoxyfenozide The reciprocal resistance of Met-R beet armyworm to chlorpyrifos, beta-cypermethrin, chlorfenapyr, tebufenozide, emamectin benzoate and other agents were measured by 1.05 ~ 2.07, there is no cross-resistance. The results of in vitro enzymatic activity assay showed that the glutathione S-transferase activity in midgut of Met-R beetle was 2.44 times of that in SSR-beetle SS group, indicating a significant difference The enhancement of the activity of cystathionine S-transferase may be related to the resistance of beet armyworm to methoxyfenozide. However, there is no significant difference in the activity of the whole esterase of Met-R beet armyworm SS with that of SS Has nothing to do with esterase.