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目的:探讨表皮生长因子(Epidermal growth factor,EGF)和口腔鳞癌侵袭转移的关系。方法:采用发色底物反应法测量不同浓度EGF作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后u-PA和PAI-1的活性,同时用Boyden Chamber观察EGF诱导口腔鳞癌TSCCa和GNM细胞转移作用。结果:不同浓度的EGF(0ng/ml,10ng/ml,20ng/ml,40ng/ml,80ng/ml)作用于两种细胞系后,随着外源性EGF浓度的增加,GNM和TSCCa细胞中u-PA和PAI-1的活性均有增加,与对照组相比差异有显著性(p<0.05);而以TSCCa细胞中u-PA和PAI-1的活性增加较为明显,u-PA和PAI-1活性与外源性EGF有剂量依赖关系,用不同浓度10ng/ml,20ng/ml,40ng/ml,80ng/ml的EGF培养TSCCa和GNM细胞2h,GNM和TSCCa细胞穿过滤膜数均有增加,在相同的浓度内,TSCCa细胞较GNM细胞增加较为明显,两者相比差异有显著性(p<0.05)。结论:EGF可促进口腔鳞癌细胞侵袭转移。
Objective: To investigate the relationship between epidermal growth factor (EGF) and invasion and metastasis of oral squamous cell carcinoma. Methods: The activity of u-PA and PAI-1 in different concentrations of EGF on oral squamous cell carcinoma TSCCa cell line and cervical lymph node metastasis GNM cell line was measured by chromogenic substrate reaction method. At the same time, EGF was used to observe the EGF-induced oral squamous cell carcinoma TSCCa and GNM cell metastasis. Results: After treated with different concentration of EGF (0ng / ml, 10ng / ml, 20ng / ml, 40ng / ml, 80ng / ml) in both cell lines, with the increase of exogenous EGF concentration, GNM and TSCCa cells The activity of u-PA and PAI-1 increased, which was significantly different from that of the control group (p <0.05). However, the increase of u-PA and PAI-1 in TSCCa cells was more obvious PAI-1 activity and exogenous EGF in a dose-dependent manner, with different concentrations of 10ng / ml, 20ng / ml, 40ng / ml of EGF EGFP cultured TSCCa and GNM cells 2h, GNM and TSCCa cells through the filter number In the same concentration, TSCCa cells increased more significantly than GNM cells, the difference was significant (p <0.05). Conclusion: EGF can promote the invasion and metastasis of oral squamous cell carcinoma.