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为了确诊海南绿橙和福橙主产区黄龙病的发生,本试验采用nested-PCR技术对94个采自琼中县和澄迈县的疑似样品进行了检测。结果表明,采自琼中县绿橙的38个样品中,有22个为阳性,带菌率为57.89%;采自澄迈县福橙的56个样品中,有10个为阳性,带菌率为17.86%。可见琼中部分地区绿橙带菌率远高于黄龙病可控上限30%,可能面临着很大的毁园风险。
In order to confirm the occurrence of the yellow dragon disease in the main producing areas of Hainan green orange and Fuzhou orange, 94 suspected samples collected from Qiongzhong County and Chengmai County were tested by nested-PCR technique. The results showed that of the 38 samples collected from Qiongzhong County green orange, 22 were positive and the carrier rate was 57.89%. Of the 56 samples collected from Chengmai Orange County, 10 were positive and the carrier rate was 17.86%. Visible in some areas of Qiongzhong green citronella rate is much higher than the upper limit of control Huanglong disease 30%, may face a great risk of destruction of the park.