为了解遗传病或复杂的基因多态现象的分子基础,需要在个体水平详细分析某些位点DNA序列的变化。目前分析每一个等位基因突变个体要经过建立基因文库、筛选、绘制物理图谱、次级克隆,DNA序列等一系列繁杂的工作。本文作者应用体外聚合酶促DNA扩增技术,对基因组DNA中特定 In order to understand the molecular basis of genetic diseases or complex genetic polymorphisms, changes in DNA sequences at certain sites need to be analyzed in detail at the individual level. The current analysis of each allele mutation individuals to go through the establishment of gene libraries, screening, mapping the physical map, secondary cloning, DNA sequences and a series of complicated work. The authors apply in vitro polymerase-DNA amplification techniques that are specific to genomic DNA