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目的研究雌性Wistar-Furth大鼠发情期不同时段垂体前叶neuregulin-1(Nrg1)的表达变化。方法采用RT-PCR观察雌性Wistar-Furth大鼠不同发情期间垂体Nrg1亚型及其内源受体ErbB-2及ErbB-4mRNA表达变化;应用Western blotting检测蛋白质水平垂体前叶及后叶Nrg1亚型表达变化。采用免疫荧光染色明确表达Nrg1的垂体细胞,并观察垂体前叶Nrg1及磷酸化ErbB-4的分布。此外,研究雄性恒河猴垂体前叶Nrg1及ErbB-4共表达。结果结果显示在大鼠动情周期Ⅰ(E1)和动情周期Ⅱ(E2),部分Nrg1亚型,尤其是Ⅲ型Nrg1mRNA表达水平较高,与E1andE2期垂体前叶Western blotting结果一致。免疫荧光染色表明促性腺激素细胞是垂体前叶Nrg1的主要来源。E1andE2期垂体前叶Nrg1分布广泛,同时伴有ErbB-4受体磷酸化激活。此外,可观察到雄性恒河猴垂体前叶部分Nrg1与ErbB-4毗邻分布。结论雌性Wistar-Furth大鼠垂体前叶有多种Nrg1亚型表达以及Nrg1/ErbB-4信号通路的存在,且与动情周期相关。在雄性非人灵长类垂体前叶存在以Nrg1/ErbB-4为基础的邻分泌机制。
Objective To investigate the expression of neuregulin-1 (Nrg1) in the anterior pituitary of female Wistar-Furth rats during different stages of estrus. Methods RT-PCR was used to observe the expression of ErbB-2 and ErbB-4 mRNA in the pituitary of female Wistar-Furth rats during different estrus periods. Western blotting was used to detect the protein levels of Nrg1 subtype in the anterior and posterior pituitary Change of expression Immunofluorescence staining of pituitary cells expressing Nrg1 was performed and the distribution of Nrg1 and phosphorylated ErbB-4 in the anterior pituitary was observed. In addition, co-expression of Nrg1 and ErbB-4 in the anterior pituitary of male rhesus monkeys was studied. The results showed that in rat estrous cycle Ⅰ (E2) and estrous cycle Ⅱ (E2), some Nrg1 subtypes, especially type Ⅲ Nrg1 mRNA expression levels higher, and E1andE2 anterior pituitary pre-Western blotting results. Immunofluorescence staining showed that gonadotrophs are the main source of Nrg1 in the anterior pituitary. E1andE2 anterior pituitary Nrg1 widely distributed, accompanied by ErbB-4 receptor phosphorylation. In addition, the adjacent distribution of Nrg1 and ErbB-4 in the anterior pituitary gland of males can be observed. Conclusion There are many Nrg1 isoforms and Nrg1 / ErbB-4 signaling in the anterior pituitary of female Wistar-Furth rats, which is related to the estrous cycle. In the male non-human primate pituitary anterior pituitary Nrg1 / ErbB-4-based mechanism of the secretion.