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目的观察厄贝沙坦是否对抗糖尿病大鼠肺损伤,探讨大鼠肺组织胶原和基质金属蛋白酶(MMPs)通路中相关因子的变化。方法雄性SD大鼠随机分为对照组(NC)、T2DM组、厄贝沙坦+糖尿病组(Ir+T2DM)。8周后测定各组FPG、TG和TC,HE和Masson染色观察肺组织形态、纤维化改变。ELISA测定肺组织MMP-2、MMP-9、TIMP-1和TIMP-2含量,Western blot检测MT1-MMP蛋白的表达。结果与NC组比较,T2DM组FPG、TG和TC水平增高,肺泡结构大部分消失,胶原增生,肺组织MMP-2、MMP-9水平下降,TIMP-1、TIMP-2水平升高,MT1-MMP蛋白表达较NC组增加(P<0.05或P<0.01)。与T2DM组比较,Ir+T2DM组肺组织形态和纤维增生改善,MMP-2、MMP-9水平增加,TIMP-1、TIMP-2水平降低,MT1-MMP蛋白表达降低(P<0.05)。结论 T2DM可诱导肺脏损伤,厄贝沙坦通过调节MMPs信号通路中的相关因子,减轻肺损伤。
Objective To observe whether irbesartan can antagonize the lung injury in diabetic rats and explore the changes of collagen and matrix metalloproteinase (MMPs) pathway in rat lung. Methods Male SD rats were randomly divided into control group (NC), T2DM group and irbesartan + diabetes group (Ir + T2DM). After 8 weeks, the levels of FPG, TG and TC in each group were determined. The morphology and fibrosis of lung tissue were observed by HE staining and Masson staining. The contents of MMP-2, MMP-9, TIMP-1 and TIMP-2 in lung tissue were measured by ELISA. The expression of MT1-MMP protein was detected by Western blot. Results Compared with NC group, the levels of FPG, TG and TC in T2DM group were higher than those in NC group, most of the alveolar structure disappeared, the collagen hyperplasia, the levels of MMP-2 and MMP-9 in the lung tissue decreased, the levels of TIMP-1 and TIMP- MMP protein expression increased compared with NC group (P <0.05 or P <0.01). Compared with T2DM group, the lung morphology and fibrosis in Ir + T2DM group were improved, the levels of MMP-2 and MMP-9 were increased, the levels of TIMP-1 and TIMP-2 were decreased and the expression of MT1-MMP was decreased (P <0.05). Conclusion T2DM can induce lung injury. Irbesartan can reduce lung injury by regulating the related factors in MMPs signaling pathway.