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目的研究血小板生成素(TPO)对人巨核细胞白血病细胞(DAMI)增殖、分化的影响。方法采用体外培养巨核细胞,将不同浓度TPO加入细胞培养液,CCK-8法检测TPO最佳刺激浓度;培养液中加入TPO,台盼蓝检测细胞增殖活力,巨核细胞乙酰胆碱脂酶染色检测细胞集落。流式细胞仪检测巨核细胞CD4l表达。结果 TPO浓度为250ng/ml时刺激作用明显。培养液中加入TPO后,巨核细胞集落计数、CD4l表达均明显增加。结论 TPO促进体外巨核细胞增殖、分化。
Objective To investigate the effect of thrombopoietin (TPO) on the proliferation and differentiation of human megakaryocytic leukemia cells (DAMI). Methods The cells were treated with different concentration of TPO in vitro and the optimum concentration of TPO was determined by CCK-8 method. TPO was added into the culture medium, the proliferation activity of the cells was detected by trypan blue and the cell colonies were detected by the method of megakaryocyte acetylcholinesterase staining . Flow cytometry was used to detect megakaryocyte CD4l expression. Results TPO concentration of 250ng / ml stimuli obvious. After addition of TPO in culture medium, the number of megakaryocyte colonies and the expression of CD4l increased significantly. Conclusion TPO can promote the proliferation and differentiation of megakaryocytes in vitro.