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目的 研究中国丙型肝炎病毒 (HCV)基因型分布。方法 应用AmplicorPCR试剂盒检测北京、青岛、固安和周口等地 2 7例抗 HCV阳性的单采浆供血员和 36例慢性丙型肝炎病人血清HCVRNA ,并对其中 36例HCVRNA阳性者用INNO LiPATM HCVⅡ试剂盒进行HCV基因分型。结果 抗 HCV阳性的单采浆供血员和慢性丙型肝炎病人HCVRNA检出率分别为 48.15 % (13/ 2 7)和 6 9.44 % (2 5 / 36 ) ,平均为 6 0 .32 % (38/ 6 3)。对 13例HCVRNA阳性的单采浆供血员和 2 3例慢性丙型肝炎病人HCV基因分型结果表明 ,2 9例 (80 .5 5 % )为 1b型 ,5例 (13.89% )为 2型 ,另 2例(5 .5 6 % )不能被分型。结论 上述调查地区流行的HCV基因型以 1b型占优势
Objective To study the distribution of hepatitis C virus (HCV) genotypes in China. Methods AmplicorPCR kit was used to detect Serum HCVRNA in 27 anti-HCV positive single plasmapheresis blood donors and 36 chronic hepatitis C patients in Beijing, Qingdao, Gu’an and Zhoukou. 36 HCVRNA positive patients were detected with INNO LiPATM HCV â ... ¢ kit for HCV genotyping. Results The detection rates of HCV RNA were 48.15% (13/27) and 6 9.44% (25/36) respectively, with an average of 60.32% (38%) in anti-HCV positive single plasma donors and chronic hepatitis C patients / 6 3). HCV genotyping of 13 cases of HCV RNA-positive apheresis blood donors and 23 cases of chronic hepatitis C patients showed that 29 (80.55%) were type 1b and 5 (13.89%) were type 2 , The other two cases (5.56%) can not be typed. Conclusion The HCV genotypes prevailing in the above survey areas are predominant in type 1b