【摘 要】
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目的:观察艾灸预处理对急性胃黏膜损伤大鼠胃黏膜细胞凋亡相关因子的影响,探讨艾灸诱导HSP70与上述因子的关系,从细胞凋亡线粒体信号转导途径探讨艾灸预处理保护胃黏膜损伤的
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目的:观察艾灸预处理对急性胃黏膜损伤大鼠胃黏膜细胞凋亡相关因子的影响,探讨艾灸诱导HSP70与上述因子的关系,从细胞凋亡线粒体信号转导途径探讨艾灸预处理保护胃黏膜损伤的机制.方法:将32只Wister大鼠随机分为4组,即捆绑组,模型组、艾灸穴位组、艾灸非穴组.艾灸穴位组和艾灸非穴组大鼠艾灸预处理8 d,捆绑组和模型组大鼠只捆绑,不艾灸.除捆绑组外,其余各组制备大鼠急性胃黏膜损伤模型.采用Western-blot方法检测大鼠胃黏膜细胞色素C(Cyt-c)的表达,免疫组织化学方法检测胃黏膜HSP70,胃黏膜细胞凋亡,Apaf-1,Caspase-9,Caspase-3,Bcl-2和Bax的表达.结果:与捆绑组相比,模型组大鼠胃黏膜HSPT0表达,胃黏膜细胞凋亡指数,胞浆Cyt-C,Apaf-1,Caspase-9,Caspase-3,Bcl-2和Bax含量明显增加(P
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