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目的:建立LC-MS/MS法测定大鼠血浆中的阿托品。方法:血浆样品经乙腈沉淀蛋白提取。采用C18色谱柱,以乙腈-0.1%甲酸(60:40,v/v)为流动相,氯氮平为内标,采用电喷雾电离源(ESI),选择正离子多离子反应监测(MRM),质核比(m/z)为290.0→123.9(阿托品)和m/z 327.1→269.9(氯氮平)。结果:阿托品在5~1000 ng/ml浓度范围内线性关系良好。方法回收率大于97%,提取回收率大于85%,日内和日间RSD小于8%。结论:方法简便、快速、准确,可满足临床药动学研究的要求。
Objective: To establish a LC-MS / MS method for the determination of atropine in rat plasma. Methods: Plasma samples were extracted by acetonitrile precipitation. C18 column was used with mobile phase of acetonitrile-0.1% formic acid (60:40, v / v) as mobile phase and clozapine as internal standard. Positive ionized multiple ion reaction monitoring (MRM) (M / z) of 290.0 → 123.9 (atropine) and m / z 327.1 → 269.9 (clozapine). Results: Atropine had good linearity in the concentration range of 5 ~ 1000 ng / ml. The recovery rate of the method was more than 97%, the extraction recovery rate was more than 85%, and the intra-day and inter-day RSD was less than 8%. Conclusion: The method is simple, rapid and accurate and can meet the requirements of clinical pharmacokinetics.