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通过比较云南烟蚜敏感品系和抗性品系的解毒酶(α-乙酸萘酯羧酸酯酶、β-乙酸萘酯羧酸酯酶)和靶标酶(乙酰胆碱酯酶)的活力,研究了烟蚜对有机磷、拟除虫菊酯和氨基甲酸酯类杀虫剂抗性的生化机制,并通过酯酶基因扩增检测和钠离子通道突变检测,研究了其抗性的分子机制。结果表明:α-乙酸萘酯羧酸酯酶活力增强是烟蚜对有机磷类、氨基甲酸酯类杀虫剂及拟除虫菊酯类杀虫剂的抗性机制之一;乙酰胆碱酯酶在烟蚜对有机磷杀虫剂抗性中起重要作用;3个抗性品系烟蚜均没有发生酯酶基因扩增,抗拟除虫菊酯品系烟蚜发生了钠离子通道突变。
By comparing the activities of detoxification enzymes (α-naphthyl acetate-6-carboxylate esterase, β-naphthyl acetate-6-carboxylate esterase) and target enzyme (acetylcholinesterase) The biochemical mechanisms of resistance to organophosphorus, pyrethroid and carbamate insecticides were investigated. The molecular mechanism of resistance was studied by esterase gene amplification and sodium ion channel mutation detection. The results showed that the enhancement of α-naphthyl acetate carboxylesterase activity was one of the mechanisms of resistance of the aphids to organophosphate pesticides, carbamates and pyrethroids. Acetylcholinesterase Which play an important role in the resistance of organophosphorus insecticides. No esterase gene amplification occurred in the three resistant strains of Myzus persicae, and sodium ion channel mutations were observed in the pyrethrum resistant pyrethroid strains.