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目的:探索大鼠膀胱Cajal间质细胞(ICC)的分离和培养方法,为进一步研究其在膀胱中的作用提供条件。方法:取大鼠的膀胱组织,采用Ⅱ型胶原酶酶解法分离细胞,将细胞悬液接种于含50ng/mlSCF、15%(v/v)FBS的DMEM培养基中,进行培养。用c-kit特异性杭体标记细胞,免疫荧光鉴定ICC细胞。结果:培养8小时后的ICC贴壁良好,并保持其固有特征:两个长的突起,多个短的侧突,胞体小,核大,c-kit抗体荧光染色阳性。结论:酶解法分离大鼠膀胱ICC并培养成功。
Objective: To explore the method of isolation and culture of Cajal interstitial cells (ICC) in rat bladder and provide the conditions for its further study in bladder. Methods: The bladder tissues of rats were taken out and the cells were isolated by the method of collagenase type Ⅱ collagenase. The cell suspension was inoculated into DMEM medium containing 50ng / ml SCF and 15% (v / v) FBS for culture. Cells were labeled with c-kit specific antibodies and ICC cells were identified by immunofluorescence. Results: After 8 hours of culture, the ICCs adhered well and maintained their inherent characteristics: two long protuberances and multiple short lateral processes with small cytoplasm and large nucleus. The positive staining of c-kit antibody was positive. Conclusion: ICC of rat bladder was separated by enzymatic method and cultured successfully.