论文部分内容阅读
本文观察了γ射线及烧伤复合伤后1 d～10d内大鼠肝线粒体MDA含量、细胞色素氧化酶、NADH-细胞色素C还原酶、CAT和SOD活力的动态变化,以反映肝线粒体内氧自由基、MDA产生和抗氧化机制的变化。结果表明线粒体MDA含量在烧伤、γ射线损伤和放烧复合伤后第2天、6天明显升高,其中烧伤组第10天有第3次高峰出现。细胞色素氧化酶和NADH-细胞色素C还原酶活力在3种损伤后有明显上升,而CAT和SOD活力在3种损伤后有明显下降。γ射线、烧伤及复合伤造成线粒体内产生O·_2~-和H_2O_2的酶活力增加,同时伴有清除O·_2~-和H_2O_2的CAT和SOD酶活力下降,这是造成线粒体膜脂质过氧化作用加速的原因之一。
In this paper, the dynamic changes of mitochondrial MDA content, cytochrome oxidase, NADH-cytochrome C reductase, CAT and SOD activity in the rat livers after 1 ~ 10 days after γ-ray and burn combined injury were observed to reflect the oxygen free Base, MDA Production and Antioxidant Mechanism Changes. The results showed that the content of MDA in mitochondria increased obviously on the 2nd day and the 6th day after the combined injury of burn, γ-ray and burn-burn, and the 3rd peak appeared on the 10th day in the burn group. Cytochrome oxidase and NADH-cytochrome C reductase activity increased significantly after three kinds of injury, while CAT and SOD activity decreased obviously after three kinds of injury. Increased activity of O · _2 ~ - and H_2O_2 in mitochondria induced by γ-rays, burns and combined injuries, accompanied by decreased activities of CAT and SOD in O · _2 ~ - and H_2O_2 -enriched mitochondrial membrane lipids Oxidation accelerated one of the reasons.