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目的 探讨血红素类物质在哮喘中的变化及与哮喘发作的关系。方法 6 0只豚鼠随机分为正常对照组、哮喘、哮喘自然缓解、地塞米松、血红素氧合酶 1(HO-1)特异性激动剂血晶素和抑制剂锡原卟啉 6组。每组均检测肺组织病理和HO-1的蛋白表达 (免疫组化染色法 ) ,应用分光光度法检测肺组织HO-1活性、血一氧化碳血红蛋白 (COHb)和NO含量 ,放射免疫竞争抑制法测定肺环磷酸鸟苷 (cGMP)。结果 哮喘组和血晶素组①肺HO-1活性 ;②血COHb ;③NO ;④肺cGMP含量与正常组比较 ,差异非常显著 (t值分别为① 5 .6 9,9.2 9,② 6 .2 8,10 .19,③ 5 .77,8.92 ,④ 9.74,6 .96 ,P <0 .0 1) ,血晶素组更为显著。肺HO-1活性 :每小时 (144 9± 42 6 ) pmol/mg ;血COHb (7.43± 2 .0 7) % ;血NO(90 .9± 16 .7) μmol/L ;肺cGMP :(1.96± 0 .6 5 ) pmol/mg ;肺有明显的嗜酸细胞浸润 ,HO-1蛋白表达≥ 4级。锡原卟啉抑制、地塞米松防治和哮喘自然缓解组各项检测指标显著下降 ,与哮喘组比较差异显著 (P <0 .0 1)。结论 哮喘时 ,体内血红素代谢产物CO和NO、降解血红素的HO-1酶类及其血红素酶类增加 ;血红素类物质增加 ,可能参与哮喘气道高反应性。
Objective To investigate the changes of hemoglobin in asthma and its relationship with asthma attacks. Methods Sixty guinea pigs were randomly divided into normal control group, asthma, spontaneous remission of asthma, dexamethasone, heme oxygenase 1 (HO-1) specific agonist hemin and inhibitor tin protoporphyrin 6 groups. The lung histopathology and HO-1 protein expression (immunohistochemical staining) were detected in each group. HO-1 activity, COHb and NO contents in lung tissue were measured by spectrophotometry, radioimmunoassay inhibition Pulmonary cyclic guanosine monophosphate (cGMP). Results ① The HO-1 activity in the asthmatic group and the hematin group were significantly higher than those in the normal group (P <0.05); ② The levels of COHb and NO in the asthmatic group were significantly higher than those in the normal group (t = 5.99, 9.29, 2 8,10 .19, ③ 5 .77,8.92, ④ 9.74,6.96, P <0.01), hematin group more significant. Lung HO-1 activity: 144 9 ± 42 6 pmol / mg per hour; blood COHb 7.43 ± 2.07%; blood NO 90.9 ± 16.7 μmol / L; lung cGMP: 1.96 ± 0. 6 5) pmol / mg; lung eosinophilic infiltration, HO-1 protein expression ≥ 4. Tin protoporphyrin inhibition, dexamethasone control and natural remission of asthma in the detection of various indicators decreased significantly compared with the asthma group (P <0. 01). Conclusions In asthma, HO-1 enzymes and heme enzymes of heme metabolites such as CO and NO, heme of heme increase, and heme substances increase, which may be involved in airway hyperresponsiveness in asthma.