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探讨转染人FasL基因的成熟树突状细胞(DC)对异体T淋巴细胞增殖和凋亡的影响,为实现临床器官移植免疫耐受提供初步实验依据.从健康成年人外周静脉血中获得成熟树突状细胞.将人FasL基因成功转染成熟树突状细胞,检测其表面分子的表达和自身凋亡情况,并对其抗原递呈功能进行分析.从异体健康成人外周血中获取T淋巴细胞,将转染成功的树突状细胞与T淋巴细胞混合培养,检测其对T淋巴细胞增殖和凋亡的影响.结果表明:人FasL基因转染没有明显影响成熟树突状细胞表面分子CD40、CD80、CD86和HLA-DR的表达;没有诱导树突状细胞自身发生凋亡;没有影响DC的抗原递呈功能.转染FasL基因后的树突状细胞使异体T淋巴细胞刺激指数明显下降,凋亡增加.因此认为,人FasL基因转染对成熟树突状细胞的表面分子表达、自身凋亡、抗原递呈等生物学性状无影响;转染FasL基因的树突状细胞使异体T淋巴细胞的增殖能力减弱,并能明显诱导T淋巴细胞凋亡.
To investigate the effects of mature dendritic cells (DCs) transfected with human FasL gene on the proliferation and apoptosis of allogeneic T lymphocytes and to provide preliminary experimental evidence for the realization of immune tolerance in clinical organ transplantation.Mature peripheral blood samples obtained from mature adult Dendritic cells.The human FasL gene was successfully transfected into mature dendritic cells to detect the expression of its surface molecules and autologous apoptosis, and to analyze the antigen presenting function of the dendritic cells.From the peripheral blood of allogeneic healthy adults, T lymphocytes Cells were transfected with dendritic cells and T lymphocytes were cultured to detect the proliferation and apoptosis of T lymphocytes.The results showed that: human FasL gene transfection did not significantly affect the maturation of dendritic cells surface molecules CD40 , The expression of CD80, CD86 and HLA-DR did not induce the apoptosis of dendritic cells themselves and did not affect the antigen presenting function of DC.The dendritic cells transfected with FasL gene significantly decreased the stimulation index of allogeneic T lymphocytes Therefore, it is considered that human FasL gene transfection has no effect on the biological characteristics such as surface molecule expression, apoptosis and antigen presentation of mature dendritic cells. The dendritic thin Cells reduced the proliferation of allogeneic T lymphocytes and induced apoptosis of T lymphocytes.