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以1份西双版纳黄瓜和2份普通黄瓜自交系为试验材料配制2个杂交组合,并构建4世代群体(P1、P2、F1、F2)。分别采用目测分级、色差仪测定a值和b值以及HPLC方法测定β-胡萝卜素含量等4个指标,研究黄瓜果肉色性状的遗传规律。结果表明:2个杂交组合F2群体的果肉色相关指标呈单峰偏态或双峰分布,表现为主基因+多基因的数量遗传特征。多世代联合分析表明,黄瓜果肉色遗传基本符合两对主基因+多基因模型。两组合8个果肉色相关指标最优模型的F2群体主基因遗传率较高,为76.0%~99.3%,多基因遗传率较低,为0~23.7%。对黄瓜橙黄果肉色的选择应在分离早期世代进行。
Two crosses were made with 1 Xishuangbanna cucumber and 2 common cucumber inbred lines. The 4-generation population (P1, P2, F1, F2) was constructed. The genetic rules of flesh color traits in cucumber were studied by visual classification, a-value and b-value determination by colorimeter and β-carotene content determination by HPLC method. The results showed that the flesh color related indicators of F2 population showed a single peak or bimodal distribution, which showed the quantitative genetic characteristics of main gene + polygene. Multiple generations of joint analysis showed that the flesh color of cucumber flesh inherited two major genes + polygene model. The two groups of eight optimal combinations of flesh-color correlation indexes had a high heritability of major gene of F2 population, ranging from 76.0% to 99.3%. The heritability of polygene was 0-23.7%. Cucumber orange yellow flesh color selection should be separated in the early generation.