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本文介绍用反相高效液相层析系统,定量测定固体制剂的炔雌醇。此法快速,重现性好,回收率97.3～101.5％,相对标准偏差0.4～2.2％。实验条件:Waters6000 A型层析仪,恒流量泵,Waters710 A型自动注射器,氙灯源萤光检测仪(激发λ=280nm、发射λ’=330nm),不锈钢柱(25cm×4.6mm内径)10μm,C-8 hydrocarbon,流动相0.05MKH_2PO_4:甲醇(2:3),流速2.0ml/min,进样量50μl,内标液邻苯二酚1.0μg/ml(流动相),标准液为炔雌醇-甲醇稀释液(25mg-100ml,吸5ml加甲醇50ml稀释)5:50,取稀释液2.00ml,加内标液5.00ml、
This article describes the quantitative determination of ethinyl estradiol in solid formulations using an RP-HPLC system. This method is rapid, reproducible, the recovery rate of 97.3 ~ 101.5%, the relative standard deviation of 0.4 to 2.2%. Experimental conditions: A Waters 6000 A chromatograph, a constant flow pump, a Waters 710 A autoinjector, a xenon lamp fluorescence detector (excitation λ = 280 nm, emission λ ’= 330 nm), a stainless steel column (25 cm × 4.6 mm id) C-8 hydrocarbon, mobile phase 0.05MKH_2PO_4: methanol (2: 3), flow rate 2.0ml / min, injection volume 50μl, internal standard solution of catechol 1.0μg / ml (mobile phase) - Methanol diluent (25mg-100ml, 5ml diluted with methanol, 5ml diluted) 5:50, take the diluent 2.00ml, add internal standard solution 5.00ml,