论文部分内容阅读
应用免疫组化ＬＳＡＢ法检测了１０５例大肠癌组织中ｐ１６蛋白。结果：大肠癌ｐ１６蛋白表达阳性率为６０．０％，与癌组织分化程度呈正相关（Ｐ＜０．０１），与癌组织浸润深度和Ｄｕｋｅｓ′分期呈负相关（Ｐ＜０．０１）。无区域淋巴结转移者的ｐ１６蛋白表达阳性率为７６．９％，伴区域淋巴结或远处器官转移者为５０．０％，二者有显著性差异（Ｐ＜０．０１）。生存超过５年者ｐ１６蛋白表达阳性率（８３．３％）明显高于５年内死亡者（５３．１％），有显著性差异（Ｐ＜０．０５）。说明ｐ１６基因能抑制大肠癌的浸润与转移，ｐ１６蛋白表达水平可作为判断大肠癌患者预后的生物学指标
Immunohistochemical LSAB method was used to detect p16 protein in 105 cases of colorectal cancer. Results: The positive rate of p16 protein expression in colorectal cancer was 60.0%, which was positively correlated with the degree of differentiation of cancer tissues (P<0.01). It was negatively correlated with the depth of tumor invasion and Dukes’ stage (P<0.01). The positive rate of p16 protein expression in patients without regional lymph node metastasis was 76.9%, with 50.0% in regional lymph nodes or distant organ metastases. There was a significant difference between them (P<0.01). The positive rate of p16 protein expression (83.3%) was significantly higher in patients who survived for more than 5 years than in those who died within 5 years (53.1%), with significant differences (P<0.05). It shows that p16 gene can inhibit the invasion and metastasis of colorectal cancer, and the expression level of p16 protein can be used as a biological indicator to determine the prognosis of colorectal cancer patients.