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采用聚丙烯酰胺凝胶电泳技术 ,对中华绒螯蟹 (Eriocheirsinensis)不同发育时期胚胎及流产胚胎的6种同工酶 (乳酸脱氢酶、醇脱氢酶、苹果酸脱氢酶、酯酶、淀粉酶和过氧化物酶 )进行了研究。结果表明 ,不同发育时期胚胎的乳酸脱氢酶、醇脱氢酶、苹果酸脱氢酶、酯酶及淀粉酶酶谱表现出一定差异。受精卵中未检测到乳酸脱氢酶同工酶活性 ,卵裂期和囊胚期出现 4条酶带 ,无节幼体及氵蚤状幼体期只有 2条酶带 ;醇脱氢酶同工酶在中华绒螯蟹胚胎发育的各阶段均有表达 ,但表达的酶带数和活性有区别 ;在受精卵和氵蚤状幼体期无苹果酸脱氢酶酶带显示 ,卵裂期酶带数最多 ,酶活性相对也最强 ,以后随着发育的进行 ,酶带数和酶活性都有减弱的现象 ;酯酶同工酶的变化较为复杂 ,特别是囊胚期 ,酯酶酶带突然全部消失 ;淀粉酶有两种类型 :α 淀粉酶和R 淀粉酶。从受精卵发育到幼体其酶带数不增反减。不同发育阶段均检测不到过氧化物酶的活性。第二次抱卵胚胎的酶活性和酶带数低于或有别于第一次抱卵的卵裂期胚胎。流产胚胎中醇脱氢酶、乳酸脱氢酶、淀粉酶与第一次抱卵胚胎不同发育时期的酶谱相比略有变化 ,而苹果酸脱氢酶、酯酶酶带数迅速减少。
The polyacrylamide gel electrophoresis (PAGE) was used to detect the activities of six isozymes (lactate dehydrogenase, alcohol dehydrogenase, malate dehydrogenase, esterase, Amylase and Peroxidase) were studied. The results showed that the lactate dehydrogenase, alcohol dehydrogenase, malate dehydrogenase, esterase and amylase zymogram of embryos at different developmental stages showed some differences. No lactate dehydrogenase isoenzyme activity was detected in fertilized eggs, and 4 bands appeared in cleavage stage and blastula stage. There were only two bands in nauplius and larval stage of larvae. Alcohol dehydrogenase isoenzyme There were differences in the number of bands and the activity of the expressed bands in embryos of Eriocheir sinensis. No bands of malate dehydrogenase were found in the zygotes, The enzyme activity is relatively the strongest, with the development of the future, the number of bands and enzyme activity have weakened phenomenon; esterase isozyme changes more complicated, especially the blastula stage, esterase with a sudden all Disappear; There are two types of amylase: alpha amylase and R amylase. From the development of fertilized eggs to the larvae of the enzyme does not increase the number of anti-subtraction. Peroxidase activity was undetectable at different developmental stages. The amount of enzyme activity and number of bands in the second somatic embryo was lower than or different from that of the first somatic embryo. The alcohol dehydrogenase, lactate dehydrogenase and amylase in aborted embryos changed slightly compared with that in the first embryo larvae, while the number of malate dehydrogenase and esterase bands decreased rapidly.