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目的:通过HPLC法测定金银花中四种主要成分绿原酸、当药苷、咖啡酸、木犀草苷的含量。方法:色谱柱为Inert-sil ODS-3 C_(18)色谱柱(250 mm×4.6 mm,5μm)。梯度洗脱法,流动相A为0.05%磷酸;B为乙腈,流速为1.0 ml·min~(-1),检测波长为234 nm。结果:绿原酸、咖啡酸在0.01～0.20 mg·ml~(-1)浓度范围内均呈良好线性关系,r=0.999 8;同时当药苷、木犀草苷在0.001～0.020 mg·ml~(-1)浓度范围内均呈良好线性关系,r=0.999 8。平均加样回收率均在96%～101%,精密度和重复性的RSD均<3%,24 h内稳定性良好。结论:本分析方法简单、灵敏、准确、重复性好,可同时分析金银花中四种主要成分。
OBJECTIVE: To determine the content of chlorogenic acid, caffeic acid and luteolin in four main components of honeysuckle by HPLC. METHODS: The column was an Inert-sil ODS-3 C 18 column (250 mm × 4.6 mm, 5 μm). Gradient elution, the mobile phase A was 0.05% phosphoric acid; B was acetonitrile, the flow rate was 1.0 ml · min -1, the detection wavelength was 234 nm. Results: There was a good linear relationship between chlorogenic acid and caffeic acid in the concentration range of 0.01 ~ 0.20 mg · ml ~ (-1), r = 0.999 8. Meanwhile, when the concentration of glycosides and luteolin was 0.001 ~ 0.020 mg · ml ~ (-1) concentration range showed a good linear relationship, r = 0.999 8. The average recovery was 96% ~ 101%, RSD of precision and repeatability were less than 3%, and the stability was good within 24 h. Conclusion: The method is simple, sensitive, accurate and reproducible. The four main components in honeysuckle can be analyzed simultaneously.