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普通野生稻具有丰富的遗传多样性,蕴藏许多有利基因.利用S5n功能性标记对来自中国14个不同居群的441份普通野生稻进行检测,发现其中18份可能携带有S5n基因,全部为杂合型,分别来自5个居群,包括广东遂溪13份,广西玉林2份,海南临高、广东高州和江西东乡各1份.进一步对这些材料S5座位可能存在的缺失及其两端DNA进行测序,发现全部材料缺失的DNA片段都与已知携带有S5n的品种02428一致,说明这18份材料确实存在S5n基因.对其中的15份材料自交一代进行基因型检测,检测到3种不同的基因型植株,其分离比(S5iS5i/S5jS5j:S5nS5i/j:S5nS5n)严重偏离1:2:1,其中S5n的杂合型和纯合型植株比例明显偏少,说明部分S5n配子可能无法正常受精.对4份代表性材料的S5座位进行全序列测定并与栽培稻比较,显示普通野生稻S5n序列出现少数碱基的差异.初步推测S5n基因在普通野生稻中已形成,属于古老的基因.对携带有S5n材料的胚囊育性进行研究,发现育性总体偏低,表明这些材料还可能存在除S5座位外决定胚囊育性其他座位的互作.
Oryza rufipogon (Oryza sativa L.) is rich in genetic diversity and contains many favorable genes.Using S5n functional markers to detect 441 common wild rice from 14 different populations in China, 18 of them may carry S5n gene, all of them are heterozygous Including 13 Guangdong Suixi, 2 Guangxi Yulin, 1 Linhai high, Guangdong Gaozhou and 1 Jiangxi Dongxiang, respectively. The possible deletions of S5 loci and their DNA at both ends Sequencing showed that all the missing DNA fragments were consistent with 02428, which is known to carry S5n, indicating that S5n was indeed found in these 18 materials.Among 15 of these materials, The segregation ratio (S5iS5i / S5jS5j: S5nS5i / j: S5nS5n) deviated significantly from 1: 2: 1 in different genotypes, and the proportion of heterozygous and homozygous plants in S5n was significantly less than that in S5nS5i / j: S5nS5n, Normal fertilization.A total of four representative S5 loci were sequenced and compared with cultivated rice, indicating that the S5n sequence of common wild rice showed a few base differences.Preliminary speculation S5n gene has been formed in common wild rice, belonging to the ancient Genes. S5n materials for carrying sac fertility study found that the overall low fertility, indicating that these materials may also be present in addition to S5 seat sac fertility decisions other seat interaction.