作者用Percoll密度梯度离心法自患者血液中分离出原虫,作为全虫颗粒抗原,免疫BALB/c小鼠,取脾细胞与SP2/0骨髓瘤细胞,进行了两批融合试验,均获成功。融合率分别为44。27及91.64％,抗体阳性率分别为9.41及19.84％。通过克隆化培养,已初步筛选出25株分泌抗人间日疟红内期原虫单克隆抗体的杂交瘤细胞。 The authors isolated the protozoa from the blood of patients using Percoll density gradient centrifugation as BALB / c mice immunized with BALB / c mice. Splenocytes and SP2 / 0 myeloma cells were obtained and two batches of fusion experiments were performed, all of which were successful. The fusion rates were 44.27 and 91.64% respectively, and the positive rates of antibodies were 9.41 and 19.84% respectively. By cloning and culturing, 25 hybridoma cells secreting monoclonal antibodies against Plasmodium vivax were initially screened.