稻瘟病菌(Magnaporthe oryzae(Hebert)Barr)是一种植物病原真菌,可引发水稻的毁灭性病害—稻瘟病,在全世界范围内造成了极大的经济损失。寻找有效防治稻瘟病的方法迫在眉睫。本课题组在前期研究中发现了一种新的稻瘟病菌致病相关基因——Mo Dam1。本研究以稻瘟病菌的RNA反转录得到的c DNA为模板克隆Dam1的ORF,并将其和p ET-30a连接,构建原核表达载体p ET-30a-Dam1,之后转化到大肠杆菌BL21(DE3)中,用IPTG诱导表达Dam1重组蛋白,最终纯化后得到Dam1蛋白。 Magnaporthe oryzae (Hebert) Barr is a phytopathogenic fungus that causes the devastating disease of rice, the rice blast, causing enormous economic losses worldwide. It is imminent to find an effective way to control rice blast. Our group found a new pathogen-related gene of Magnaporthe grisea - Mo Dam1. In this study, we cloned the ORF of dam1 with c DNA reverse transcribed from RNA of M. grisea and ligated it with p ET-30a to construct prokaryotic expression vector p ET-30a-Dam1 which was then transformed into E. coli BL21 ( DE3), Dam1 recombinant protein was induced by IPTG, and Dam1 protein was finally purified.