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目的:探讨食管鳞癌组织中p53、Ki-67及增殖细胞核抗原(PCNA)的表达与食管癌患者放疗近期疗效及预后的关系。方法:收集接受放疗的食管癌患者83例,采用免疫组化方法检测活检癌组织中p53、Ki-67及PCNA的表达,并分析其与临床病理特征、放疗疗效和预后的关系。结果:p53、Ki-67及PCNA在食管鳞癌中高表达率分别为57.8%、72.3%、67.5%。p53、Ki-67及PCNA表达与性别、年龄、侵犯部位、肿瘤长度等各临床病理因素无关(P>0.05),p53与肿瘤分化程度、临床分期、淋巴结转移、放疗近期疗效明显相关(P<0.05),Ki-67与肿瘤分化程度、淋巴结转移、放疗近期疗效明显相关(P<0.05),PCNA与临床分期、淋巴结转移、放疗近期疗效明显相关(P<0.05)。p53低、高表达组食管癌患者3年总生存率分别为57.14%、27.08%。结论:p53、Ki-67及PCNA可作为评价食管癌恶性程度、放射敏感性的生物学指标,p53可作为评价食管癌预后的有效指标。
Objective: To investigate the relationship between the expression of p53, Ki-67 and proliferating cell nuclear antigen (PCNA) in esophageal squamous cell carcinoma and the short-term efficacy and prognosis of patients with esophageal cancer. Methods: Eighty-three patients with esophageal cancer who underwent radiotherapy were recruited. The expressions of p53, Ki-67 and PCNA were detected by immunohistochemistry and their relationship with clinicopathological characteristics, radiotherapy and prognosis were analyzed. Results: The high expression rates of p53, Ki-67 and PCNA in esophageal squamous cell carcinoma were 57.8%, 72.3% and 67.5% respectively. The expressions of p53, Ki-67 and PCNA had no correlation with the clinicopathologic factors such as gender, age, site of invasion and tumor length (P> 0.05). The correlation between p53 and tumor differentiation, clinical stage, lymph node metastasis and radiotherapy was significant 0.05). There was a significant correlation between the expression of Ki-67 and the degree of tumor differentiation, lymph node metastasis and radiotherapy (P <0.05). There was significant correlation between PCNA and clinical stage, lymph node metastasis and radiotherapy (P <0.05). The 3-year overall survival rates of patients with low and high expression of esophageal cancer were 57.14% and 27.08%, respectively. Conclusions: p53, Ki-67 and PCNA can be used as biomarkers to evaluate the malignant degree and radiosensitivity of esophageal cancer, and p53 can be used as an effective index to evaluate the prognosis of esophageal cancer.