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目的:通过去氢骆驼蓬碱 (HM)微球与羟基喜树碱 (HCPT)注射液对大鼠正常肝细胞 (BRL )的毒性作用的比较研究 ,为 HM的临床应用提供佐证。方法:采用 MTT法测试一定浓度范围内 HM与 HCPT对大鼠正常肝细胞 (BRL)的细胞毒作用大小。 结果 :在一定浓度范围内 ,HM与 HCPT的细胞毒作用与药物浓度均呈线性关系 ;针对 BRL 细胞 ,在一定的浓度范围内 HM的细胞毒作用较 HCPT注射液的细胞毒作用为小 ,经检验差别有统计学意义 (P <0 .0 1)。结论:MTT法操作简单、快速、敏感 ,是较好的体外实验方法之一。HM微球新剂型对 BRL的毒性作用小于 HCPT注射液 ,体外实验表明其毒副作用小 ,结合其剂型的靶向性、缓释性、栓塞性等特点 ,有潜在的临床应用价值
OBJECTIVE: To compare the toxic effects of dehydrohalacium (HM) microspheres and hydroxycamptothecin (HCPT) injection on rat normal liver cells (BRL), and to provide evidence for the clinical application of HM. Methods: The cytotoxic effects of HM and HCPT on rat normal liver cells (BRL) were tested by MTT assay. Results: The cytotoxicity of HM and HCPT showed a linear relationship with drug concentration in a certain concentration range. For BRL cells, cytotoxicity of HM was smaller than that of HCPT injection in a certain concentration range. Test difference was statistically significant (P <0. 01). Conclusion: MTT assay is simple, rapid and sensitive and is one of the best experimental methods in vitro. The toxic effects of the new HM microspheres on BRL were less than that of HCPT injection. The in vitro experiments showed that the toxicities and side effects of HM microspheres were small. Combined with its targeting characteristics, sustained release and embolism, the potential therapeutic value of HM microspheres has potential clinical value