论文部分内容阅读
目的:探讨在束缚-浸水应激状态下,情绪变化、体温降低、胃肠道的反馈信息三者中哪种因素是诱发延髓、下丘脑神经元Fos表达的主导因素。方法:将雄性Wistar大鼠随机分两组:手术组、对照组。手术组切断双侧膈肌下迷走神经,对照组同样暴露神经但不切断。术后6d进行实验,将大鼠束缚-浸水应激3h后处死,测腹腔温度;取胃,观察胃粘膜损伤程度;取脑,应用免疫组化染色方法观察两组动物延髓、下丘脑神经元的Fos表达。结果:手术组动物延髓迷走神经运动背核、孤束核、下丘脑室旁核Fos表达较对照组减弱(P<0.05),延髓最后区减弱最为显著(P<0.01);腹腔温度低于对照组(P<0.05);胃粘膜损伤程度较对照组减轻(P<0.05)。结论:研究表明,束缚-浸水应激过程中,诱发延髓迷走神经背核、孤束核、最后区、下丘脑室旁核神经元Fos表达的主要因素是胃肠的反馈信息。
OBJECTIVE: To explore the factors that affect the expression of Fos in medulla oblongata and hypothalamic neurons in the state of restraint-immersion stress, emotional changes, hypothermia and gastrointestinal feedback. Methods: Male Wistar rats were randomly divided into two groups: operation group and control group. Surgical group cut bilateral vagus nerve vagus nerve, the control group also exposed nerve but not cut off. After 6 days, rats were restrained - immersed in water for 3 hours and then sacrificed to measure intraperitoneal temperature. The gastric cavity was taken and the degree of gastric mucosal damage was observed. The brains were sacrificed and the brains of the medulla oblongata and hypothalamic neurons were observed by immunohistochemical staining. Fos expression. Results: The expression of Fos in medullas, nucleus tractus of solitary tract and paraventricular nucleus of hypothalamus in the operation group was weaker than that in the control group (P <0.05), and the final area of the medulla oblongata was the weakest (P <0.01). The intra-abdominal temperature was lower than that in the control group (P <0.05). The degree of gastric mucosal damage was lower than that of the control group (P <0.05). CONCLUSIONS: The study shows that the main factor that induces Fos expression in the medulla dorsal vagal nucleus, nucleus tractus solitarius, caudal region and hypothalamic paraventricular nucleus during the binding-immersion stress is the gastrointestinal feedback.