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为了明确重金属Cd2+对拟水狼蛛Pirata subpiraticus体内金属硫蛋白(metallothionein,MT)含量及其生长发育的影响,在室内条件下用5个不同浓度(0,10,20,40和80μg/g)的Cd2+培养的黑腹果蝇Drosophila melanogaster饲喂从5种不同生境下(S1,S2,S3,S4和S5)采集的拟水狼蛛性成熟雌蛛产卵孵化的幼蛛,待幼蛛性成熟后取所得雌蛛成蛛采用原子吸收光谱法测定了Cd2+诱导下拟水狼蛛体内金属硫蛋白含量及其存活率和生长率。结果表明:食物中过量的Cd2+能够通过食物链进行传递并在拟水狼蛛体内积累,积累量随黑腹果蝇培养基中Cd2+浓度的增加而增加,存在显著的浓度-效应关系。不同浓度的Cd2+能够诱导拟水狼蛛体内MT不同的表达,表达量与Cd2+浓度显著正相关(P<0.05)。当浓度低于20μg/g时,污染点(S1,S2,S3和S4)拟水狼蛛体内MT表达量显著高于参照组S5(P<0.05);当高于20μg/g时,所有样点拟水狼蛛体内MT表达量差异不显著(P>0.05)。拟水狼蛛存活率和成长率随着Cd2+浓度的升高呈下降趋势。据此认为,金属硫蛋白可能是蜘蛛耐受重金属污染的重要机制,与重金属具有一定浓度-效应关系。
In order to clarify the effect of heavy metal Cd2 + on metallothionein (MT) content and its growth in Pirata subpiraticus, five different concentrations (0, 10, 20, 40 and 80 μg / g) Cd2 + cultured Drosophila melanogaster was fed on spawning larvae of sexual maturity female spiders collected from five different habitats (S1, S2, S3, S4 and S5) After harvesting, the obtained female spider was used to determine the content of metallothionein and the survival rate and growth rate of the spider under the Cd2 + induction by atomic absorption spectrometry. The results showed that excessive Cd2 + in food could be transmitted through the food chain and accumulated in the body of Pirata subpiraticus. The accumulation increased with the increase of Cd2 + concentration in Drosophila melanogaster, and there was a significant concentration-effect relationship. Different concentrations of Cd2 + can induce different expression of MT in the body of Pirata sublica. The expression of Cd2 + was significantly and positively correlated with Cd2 + concentration (P <0.05). When the concentration was lower than 20μg / g, the expression level of MT in the contaminated spots (S1, S2, S3 and S4) was significantly higher than that in the reference group S5 (P <0.05); when the concentration was higher than 20μg / g, There was no significant difference in MT expression between the two groups (P> 0.05). The survival rate and growth rate of Pirata subpiraticus showed a downward trend with the increase of Cd2 + concentration. Therefore, metallothionein may be an important mechanism of spider tolerance to heavy metal pollution, and has a certain concentration-effect relationship with heavy metals.