论文部分内容阅读
目前已知,性甾体激素在调节LHRH分泌及其在LH、FSH分泌的过程中起着重要作用。在垂体,从LHRH与其受体结合至最终两种促性腺激素的释放都可能是性甾体激素的作用部位。为确定该种激素是否对垂体LHRH受体有直接作用,作者观察了LHRH高效类似物~(125)I-〔D-丝(丁叔)~6〔去甘~(10)〕-LHRH乙基酰胺与离体培养大鼠垂体前叶细胞的特异性结合以及双氢睾丸酮对LHRH受体的可能作用。实验用离体培养的雌性大鼠垂体前叶细胞,每毫升培养液含7.5×10~5个细胞,使用~(125)Ⅰ标记的〔D-丝(丁叔)~6〕〔去甘~(10)〕-LHRH乙基酰胺,以放射受
It is known that sex steroid hormones play an important role in the regulation of LHRH secretion and its secretion in LH and FSH. In the pituitary, the release of LHRH from its receptor to both final gonadotropins may be the site of action of the sex steroid hormone. To determine whether this hormone has a direct effect on the pituitary LHRH receptor, the authors observed the effect of the LHRH analogues ~ (125) I- [D- Specific binding of amides to anterior pituitary cells in vitro and possible effects of DHEA on LHRH receptors. Experimental use of cultured rat anterior pituitary cells, per ml of culture medium containing 7.5 × 10 ~ 5 cells using ~ (125) Ⅰ labeled 〔D-wire (Dingshu) ~ 6〕〕 〔to Gan ~ (10)] - LHRH ethylamide to radiation