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目的研究脑胶质细胞瘤病人外周血中白细胞介素2(IL-2)、可溶性白细胞介素2受体(sIL-2R)的表达以及红细胞免疫和T淋巴细胞亚群的变化规律,探讨它们之间的相互关系。方法对55例脑胶质瘤病人及55例健康献血员,采用酶联免疫吸附(ELISA)法测定血清IL-2s、IL-2R含量,免疫黏附法测定红细胞免疫活性及其调节功能,链亲和素-过氧化物酶(SP)一步法测定CD3、CD4、CD8细胞数。结果脑胶质细胞瘤组IL-2含量较对照组显著性降低(P<0.01),sIL-2R则显著性升高(P<0.01);红细胞C3b受体(RBC-C3bR)、红细胞免疫调节促进因子(RFER)亦显著性降低(P<0.01),而红细胞免疫复合物(RBC-ICR)、红细胞免疫调节抑制因子(RFIR)则显著性升高(P<0.01);CD3、CD4细胞数显著性降低(P<0.001),而CD8无显著性差异(P>0.05)。结论脑胶质细胞瘤病人存在免疫功能低下;检测血清IL-2和sIL-2R含量、红细胞免疫功能及T细胞亚群活性,对脑胶质细胞瘤病人的免疫机制研究具有重要意义。
Objective To study the expression of interleukin 2 (IL-2), soluble interleukin 2 receptor (sIL-2R) and the changes of erythrocyte immunity and T lymphocyte subsets in peripheral blood of patients with glioma, The relationship between. Methods Serum levels of IL-2s and IL-2R were measured by enzyme-linked immunosorbent assay (ELISA) in 55 glioma patients and 55 healthy blood donors. The immune activity of erythrocytes and its regulatory function were determined by immunoadsorption assay. And superoxide dismutase (SP) were used to determine the number of CD3, CD4 and CD8 cells. Results The level of IL-2 in glioma group was significantly lower than that in control group (P <0.01), while sIL-2R was significantly increased (P <0.01). Red blood cell C3b receptor (RBC-C3bR) (RBC-ICR) and erythrocyte immunoregulatory inhibitory factor (RFIR) were significantly increased (P <0.01). The number of CD3 and CD4 cells Significantly decreased (P <0.001), while CD8 no significant difference (P> 0.05). Conclusions There are immunocompromised patients with glioma. The detection of serum IL-2 and sIL-2R levels, erythrocyte immune function and T lymphocyte subsets activity are of great significance for the study of immune mechanism in patients with glioma.