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目的:观察清宫汤对高架十字迷宫小鼠行为学及脑神经递质的影响,探讨其抗焦虑作用及机制。方法:将雄性小鼠随机分为空白对照组(生理盐水)、地西泮对照组(0.001 5 g.kg-1)、清宫汤高、中、低剂量组(12,6,3 g.kg-1),每组10只。灌胃给药,每天1次,连续10 d后,采用高架十字迷宫焦虑动物模型,观察清宫汤对小鼠行为学的影响,酶联免疫吸附测定法(ELISA)检测小鼠脑神经递质含量。结果:清宫汤明显提高小鼠在高架十字迷宫装置中的开放臂进入次数比(OE%)和开放臂停留时间比(OT%);与地西泮组比较,清宫汤高剂量组OE%有显著差异(P<0.01),低剂量组OT%有显著差异(P<0.05)。脑神经递质测试,清宫汤明显降低5-羟色胺(5-HT),去甲肾上腺素(NE)浓度,其中中剂量组5-HT浓度降低显著(P<0.05),高剂量组NE浓度降低显著(P<0.01);与地西泮组比较,清宫汤各组5-HT,NE浓度无显著差异;多巴胺(DA)浓度各组无统计学差异。结论:清宫汤具有抗焦虑作用,其作用机制与降低小鼠脑组织中5-HT,NE浓度相关。
Objective: To observe the influence of Qing Gong Tang on the behavior and neurotransmitter in elevated maze of mice and to explore its anti-anxiety effect and its mechanism. Methods: Male mice were randomly divided into blank control group (saline), diazepam control group (0.001 5 g.kg-1), Qing Gong Tang high, medium and low dose groups -1), each group of 10. The rats were administered intragastrically once a day for 10 consecutive days. The model of elevated masculine maze anxiety was used to observe the effect of Qinggong Decoction on mice behavior. The content of neurotransmitter in the brain of mice was measured by ELISA. . Results: Qing Gong Decoction significantly increased the ratio of open arm entry (OE%) and open arm residence time (OT%) in the elevated plus-maze device. Compared with the diazepam group, Significant difference (P <0.01), low dose OT% significant difference (P <0.05). The brain neurotransmitter test showed that Qing Gong Decoction significantly decreased the concentrations of 5-HT and NE, especially in the medium dose group (P <0.05) and in the high dose group (P <0.01). Compared with diazepam group, the concentration of 5-HT and NE in each group of Qinggong Decoction had no significant difference. There was no significant difference in each concentration of DA. Conclusion: Qing Gong Tang has anti-anxiety effects and its mechanism of action is related to decreasing the concentration of 5-HT and NE in mouse brain tissue.